T cell costimulatory molecule, inducible costimulator (ICOS)/B7-related protein-1 (B7RP-1, B7-H2, GL50, ICOS-L) is a ligand for the ICOS receptor that initiates T and B cell proliferation and cytokine secretion.
B7RP-1 interactions play an essential role in T cell dependent B cell activation in peripheral lymphoid organs such as spleen and lymph nodes.
B7RP-1 protein is present in myeloid leukocytes and by Northern blot there are 2.4, 3.0 and 7.0kb transcripts in brain, heart, kidney and liver, with lower expression in colon and thymus and a 1.1kb transcript in leukocytes.
Tumor necrosis factor å (TNFalpha), granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin 4 (IL-4) enhance B7RP-1 expression.
LPS induced up regulation of B7RP-1 is dependent on the MyD88 dependent signaling pathway.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human ICOSLG. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to full length human ICOSLG.
