84-4388-79　［取扱停止］HYAL2 (Hyaluronidase-2, Hyal-2, Hyaluronoglucosaminidase-2, Lung Carcinoma Protein 2, LuCa-2, LUCA2) 50ul　360440

特徴

• Hyaluronidases (HAases or HYALs) are a family of lysosomal enzymes that are crucial for the spread of bacterial infections and of toxins present in a variety of venoms.
• HYALs may also be involved in the progression of cancer.
• In humans, six HYAL proteins have been identified.
• HYAL proteins use hydrolysis to degrade hyaluronic acid (HA), which is present in body fluids, tissues and the extracellular matrix of vertebrate tissues.
• HA keeps tissues hydrated, maintains osmotic balance and promotes cell proliferation, differentiation and metastasis.
• HA is also an important structural component of cartilage and other tissues and acts as a lubricant in joints.
• HYAL2 is a 452aa peptide that localizes to the lysosomes of mammalian cells.
• The hyaluronidase activity of HYAL2 is most efficient at a pH below 4, and it only hydrolyzes HAs of high molecular mass.
• HYAL2 is also the receptor for two exogenous oncogenic viruses, Jaagsiekte sheep retrovirus (JSRV) and Enzootic nasal tumor virus.
• The viral envelope of JSRV physically associates with and activates HYAL2, consequently activating the Akt1 and mitogen-activated protein kinase-1 pathways, leading to oncogenic transformation.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.