Hyaluronidases (HAases or HYALs) are a family of lysosomal enzymes that are crucial for the spread of bacterial infections and of toxins present in a variety of venoms.
HYALs may also be involved in the progression of cancer.
In humans, six HYAL proteins have been identified.
Most HYAL proteins degrade hyaluronic acid (HA), which is present in body fluids, tissues and the extracellular matrix of vertebrate tissues.
HA keeps tissues hydrated, maintains osmotic balance and promotes cell proliferation, differentiation and metastasis.
HA is also an important structural component of cartilage and other tissues and acts as a lubricant in joints.
HYAL1 is a 435aa hyaluronidase that is is expressed in multiple tissues, specifically in the serum, and is not expressed in brain.
HYAL1 degrades HA into into fragments that stimulate angiogenesis.
Expression of HYAL1 in various cancer cells may have a role in the regulation of tumor growth and progression.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human HYAL1.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human HYAL1.
