Pre-mRNA splicing is a critical step in the posttranscriptional regulation of gene expression.
Heterogeneous nuclear ribonucleoprotein Q (hnRNP Q) is involved in RNA processing and is necessary for efficient pre-mRNA splicing.
hnRNP is widely expressed and developmentally regulated.
hnRNP Q interacts with survival motor neuron protein (SMN).
Loss of function of SMN results in spinal muscular atrophy, a common neurodegenerative disease.
The most common deletion in SMN genes disrupts the interaction between SMN and hnRNP Q.
hnRNP Q is upregulated after midnight, and this upregulation correlates with an abrupt decline in AANAT, the key enzyme in melatonin synthesis.
Rhythmic AANAT mRNA degradation mediated in part by hnRNP Q implicates this enzyme in the regulation of circadian oscillation.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:50-:200Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human hnRNP Q. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human hnRNP Q.
