84-4387-83　［取扱停止］HAS3 (Hyaluronan Synthase 3, Hyaluronate Synthase 3, Hyaluronic Acid Synthase 3, HA Synthase 3, HAS3) 100ul　360386

特徴

• HAS1, HAS2 and HAS3 are HA (hyaluronan or hyaluronic acid) synthase proteins.
• The extracellular matrix in most vertebrates express HA, which is a high molecular weight linear polysaccharide composed of alternating glucuronic acid and N-acetylglucosamine residues linked by b-1,3 and b-1,4 glycosidic bonds.
• The three HAS genes show distinct patterns of expression during development and their protein products play significantly different roles in the formation of the HA matrix.
• Both HAS1 and HAS2 synthesize high molecular-weight HA, whereas HAS3 produces lower molecular weight HA.
• The expression of the three HAS isoforms is more prominent in growing cells than in resting cells and is differentially regulated by various stimuli, suggesting distinct functional roles of the three proteins.
• HAS3 produces both secreted and cell-associated forms of hyaluronan and is the most active of the three isoforms of this enzyme in adults.
• HAS3 gene expression plays a crucial role in the regulation of hyaluronan synthesis in the epidermis.
• Specifically, IFN-g markedly upregulates HAS3 mRNA, whereas TGFbeta downregulates HAS3 transcript levels.
• The human HAS3 gene maps to chromosome 16q22.1.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.