Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist.
This phenomenon, referred to as agonistmediated desensitization, involves phosphorylation of the receptor by two classes of enzymes.
The first are the second messenger-regulated kinases such as c-AMP dependent protein kinase A and protein kinase C.
The second are the G protein-coupled receptor kinases (GRKs).
At least seven members of the GRK family have been identified.
These include rhodopsin kinase, GRK 1; two forms of B-adrenergic receptor kinase, GRK 2 (BARK, BARK1) and GRK 3 (BARK2); IT-11 (GRK 4); GRK 5, GRK 6 and GRK 7.
Phosphorylation of receptors by GRKs appears to be strictly dependent on the receptor being in its agonist-activated state.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:50-1:200Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human GRK6. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human GRK6.
