GPD2 (glycerol-3-phosphate dehydrogenase 2, mitochondrial), also known as GDH2 or GPDM, is a 727aa protein belonging to the FAD-dependent glycerol-3-phosphate dehydrogenase family.
GPD2 is involved in the conversion of glycerol-3-phosphate (G-3-P) to dihydroxyacetone phosphate (DHAP) while reducing enzyme-bound FAD.
Localizing to the outer surface of the inner mitochondrial membrane, GPD2 acts in conjunction with GPD1 (a cytosolic NAD-linked GPD) to form a glycerol phosphate shuttle that ultimately results in the reoxidation of NADH formed during glycolysis.
While widely expressed in adult and fetal tissue, GPD2 is found at highest levels in human pancreatic islets where it is essential for pancreatic B-cell glucose-sensory function.
Decreased levels of GPD2 leads to impaired glucose-stimulated insulin release in noninsulin-dependent diabetes mellitus.
Existing as two alternatively spliced isoforms, GPD2 contains two EF-hand domains and maps to human chromosome 2q24.1.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human GPD2. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human GPD2.
