84-4386-97　［取扱停止］GORASP1 (Golgi Reassembly-stacking Protein 1, Golgi Peripheral Membrane Protein p65, Golgi Phosphoprotein 5, GOLPH5, Golgi Reassembly-stacking Protein of 65kD, GOLPH5, GRASP65) 50ul　360333

特徴

• The Golgi apparatus is a highly complex organelle comprised of a stack of cisternal membranes on the secretory pathway from the ER to the cell surface.
• The structure is maintained by an exoskeleton or Golgi matrix constructed from a family of coiled-coil protein, the golgins and other peripheral membrane components such as GRASP55 and GRASP65.
• GRASP55 (Golgi reassembly stacking protien or p59) is a component of the Golgi stacking machinery.
• GRASP55 is highly homologous to GRASP65 and contains two PDZ domains.
• GRASP55 is myristoylated and palmitoylated.
• Unlike GRASP65, GRASP55 does not have detectable binding with the vesicle docking protein GM130 and is located on the medial-Golgi rather than cis-Golgi.
• Both GRASP55 and GRASP65 function in the stacking of Golgi cisternae.
• The novel coiled-coil protein golgin 45 interacts with GRASP55 and the GTP form of Rab 2, suggesting that GRASP55 and golgin 45 form a Rab 2 effector complex on medial-Golgi essential for normal protein transport and Golgi structure.
• ERK2 directly phosphorylates GRASP55, which is phosphorylated in mitotic cells, suggesting that mitogen-activated protein kinase kinase (MKK)/ERK pathway phosphorylates the Golgi during mitosis.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.