Glycine N-methyltransferase (GNMT) is a 295aa protein that catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy).
This process indicates that GNMT probably plays a crucial role in the regulation of tissue concentration of AdoMet and in the metabolism of methionine.
Originally identified as a methyl donor, AdoMet is now considered a key metabolite that regulates hepatocyte growth, death and differentiation.
Biosynthesis of AdoMet occurs in all mammalian cells as the first step in methionine catabolism in a reaction catalyzed by methionine adenosyltransferase (MAT).
Decreased hepatic AdoMet biosynthesis is a consequence of all forms of chronic liver injury.
In chronic liver AdoMet deficiency, the liver is predisposed to further injury and can develop spontaneous steatohepatitis and hepatocellular carcinoma.
However, impaired AdoMet metabolism, which occurs in patients with mutations of GNMT, can also lead to liver injury.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human GNMT. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human GNMT.
