Granulysin (NKG5) is a saposin-like protein (SAPLIP) that is expressed in granular cells and functions as an effector molecule for antimicrobial responses in the hematopoietic system.
Upon antigen stimulation generated from intracellular pathogens such as bacteria, fungi, and parasites, granulysin mediates the release of cytotoxic granules from Cytolytic T lymphocytes (CTLs) and Natural killer cells (NKs), a process that can lead to apoptosis.
The potent antimicrobial activity of granulysin may occur through elevated pathogen host-cell permeability and lysis.
Human granulysin is expressed as proteins of two sizes derived from three unique transcripts.
Granulysin expressing CTLs, in the presence of perforin, can kill extracellular and intracellular Mycobacterium tuberculosis through a granule-dependent mechanism, suggesting that granulysin may play a broad role in T cell mediated host protection against certain pathogens.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human GNLY.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human GNLY.
