GLUD1 (glutamate dehydrogenase 1), also known as GDH, GDH1 or GLUD, and GLUD2 (glutamate dehydrogenase 2), also known as GDH2 or GLUDP1, are both mitochondrial matrix enzymes belonging to the Glu/Leu/Phe/Val dehydrogenases family.
Exisiting as homohexamers, GLUD1 catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia while GLUD2 is involved in the recycling of glutamate during neurotransmission.
GLUD1 is critical for regulating aa induced insulin secretion and is allosterically activated by ADP and inhibited by GTP and ATP.
Mutations in the gene encoding GLUD1 causes hyperinsulinism- hyperammonemia syndrome (HHS), which is an inherited condition characterized by high insulin and ammonia levels in the blood.
GLUD1 may also be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate.
GLUD2 is expressed in testis and retina, with lower levels found in brain.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human GLUD2. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human GLUD2.
