Glutaredoxins, or thioltransferases, are cytoplasmic proteins included in the glutaredoxin family.
Human mitochondrial glutaredoxin (Grx2) is the first ironsulfur containing protein discovered in the glutaredoxin family of proteins.
Instrumental in maintaining the redox environment of the cell, Grx2 maintains redox homeostasis specifically within the mitochondrion by allowing the ironsulfur cluster to function as a redox target for the activation of Grx2 during conditions of stress, when free radicals are formed.
Once oxidative stress induces apoptosis in a cell, Grx2 takes action by reducing low molecular weight proteins and disulfides.
Any time that NADPH and Glutathione Reductase reside in the cell, Grx2 modulates glutathione-disulfide oxidoreductase activity.
The gene that encodes human Grx2 consists of three introns and four exons, and is 10 kilobases long.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human GLRX2.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human GLRX2.
