84-4386-49　［取扱停止］GFI1B (Zinc Finger Protein Gfi-1b, Growth Factor Independent Protein 1B, Potential Regulator of CDKN1A Translocated in CML) 50ul　360303

特徴

• Growth factor independent 1 (Gfi-1) is a transcriptional repressor that specifically binds to the DNA consensus sequence TAAATCAC(A/T)GCA.
• The carboxy-terminus of Gfi-1 contains six C2H2-type zinc finger motifs, and zinc fingers 3, 4 and 5 are required for the binding of Gfi-1 to its DNA binding site.
• Gfi-1 also contains a 20aa SNAG domain which mediates transcriptional repression.
• It represses Bax at the mRNA and protein levels, resulting in the inhibition of cell death.
• Gfi-1 is expressed outside the lymphoid system in granulocytes and activated macrophages.
• Gfi-1B, a related protein, is a transciptional repressor primarily expressed in bone marrow and spleen.
• Gfi-1B is a direct repressor of the p21 promoter and the SOCS-1 and -3 promoters.
• The genes encoding human Gfi-1 and Gfi-1B map to chromosome 1p22 and 9q34.13, respectively.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.