Glucokinase (also designated hexokinase IV, HXKIV or GCK) plays a key role in the regulation of glucose-induced insulin secretion.
GCK is expressed in pancreatic beta cells where it functions as the major glucose sensor of the body, determining the “set point” for insulin secretion.
GCK is also expressed in the liver, where it catalyzes the first committed step in the disposal of glucose.
Phosphorylation of glucose by glucokinase appears to be the ratelimiting step for glucose catabolism.
A lack of glucokinase activity leads to reduced insulin secretion and hyperglycemia, and has been implicated as a cause for maturity onset diabetes of the youth (MODY).
In fact, heterozygous point mutations in the gene encoding GCK have been detected in individuals suffering from MODY.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:50-1;200Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human GCK. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human GCK.
