GBP1 (guanylate binding protein 1) is a 592aa protein member of the GTPase protein family and is able to bind specifically to guanine nucleotides such as GMP, GDP and GTP.
GMP is hydrolyzed to GTP in two consecutive cleavage step, both of which are carried out by GBP1.
Localized to the cytoplasm, GBP1 is expressed in endothelial cells of the vascular system and is induced by IFN-g during macrophage induction.
GBP1 is thought to regulate the expression of MMP-1, which mediates the proliferation and invasiveness of endothelial cells.
GBP1 plays a key role in regulating inflammatory cytokines and provides protection against vesicular stomatitis and encephalomyocarditis viruses.
GBP1 expression is highly induced in the vessels of skin diseases such as psoriasis and Kaposi’s sarcoma, making it a novel cellular activation marker that characterizes inflammatory cytokines of endothelial cells.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:50-1:200Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human GBP1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human GBP1.
