84-4386-28　［取扱停止］GATA3 (Trans-acting T-Cell-specific Transcription Factor GATA-3, GATA-binding Factor 3) 100ul　360292

特徴

• Members of the GATA family share a conserved zinc finger DNA-binding domain and are capable of binding the WGATAR consensus sequence.
• GATA-1 is erythroid-specific and is responsible for the regulated transcription of erythroid genes.
• It is an essential component in the generation of the erythroid lineage.
• GATA-2 is expressed in embryonic brain and liver, HeLa and endothelial cells, as well as in erythroid cells.
• Studies with a modified GATA consensus sequence, AGATCTTA, have shown that GATA-2 and GATA-3 recognize this mutated consensus while GATA-1 has poor recognition of this sequence.
• This indicates broader regulatory capabilities of GATA-2 and GATA-3 than GATA-1.
• GATA-3 is highly expressed in T lymphocytes.
• GATA-4, GATA-5 and GATA-6 comprise a subfamily of transcription factors.
• Both GATA-4 and GATA-6 are found in heart, pancreas and ovary; lung and liver tissues exhibit GATA-6, but not GATA-4 expression.
• GATA-5 expression has been observed in differentiated heart and gut tissues and is present throughout the course of development in the heart.
• Although expression patterns of the various GATA transcription factors may overlap, it is not yet apparent how the GATA factors are able to discriminate in binding their appropriate target sites.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000 Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.