CRIF1, also known as GADD45GIP1, PLINP1, PRG6 or CKBBP2, is a 222aa nuclear protein that plays a role in apoptosis control.
Expressed in a variety of tissues, including heart, thyroid, trachea, kidney, ovary, pancreas, testis and stomach, CRIF1 functions as a negative regulator of G1 to S phase cell cycle production, specifically by working with GADD 45 proteins to inhibit the activity of cyclin-dependent kinases (Cdks).
While overexpression of CRIF1 results in cell cycle arrest at the G1 phase, downregulation of CRIF1 by p53 in apoptotic cells promotes cell cycle progression and may be an important factor in tumor growth and metastasis.
CRIF1 is subject to phosphorylation by casein kinase II, an event that is thought to decrease CRIF1 activity and promote cellular proliferation.
Human CRIF1 shares 90% homology with its mouse counterpart, suggesting a conserved role between species.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes human GADD45GIP1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human GADD45GIP1.
