The frizzled gene, originally identified in Drosophila melanogaster, was shown to be involved in the development of tissue polarity.
The mammalian homolog of frizzled as well as several secreted mammalian frizzled-related proteins, FRP-1 (also designated SARP2), FRP-2 (also designated SARP1), FRP-3, FRP-4 and SARP3 (also designated FRP-5), have been identified.
The Frizzled proteins contain seven transmembrane domains, a cysteine-rich domain in the extracellular region and a C-terminal Ser/Thr-xxx-Val motif, and they function as receptors for Wnt.
The Frizzled-1 gene maps to human chromosome 7q21 and is expressed in adult heart, placenta, lung, kidney, pancreas, prostate and ovary and in fetal lung and kidney.
Frizzled-2 is expressed in adult heart and fetal brain, lung and kidney.
The frizzled related proteins FRP-1, FRP-2, FRP-3, FRP-4 and SARP3 are secreted proteins of approximately 30kD that contain regions of homology to the cysteine-rich ligand-binding domain of frizzled and a conserved hydrophilic C-terminal.
The gene encoding human SARP3 maps to chromosome 4q31.3 and is expressed in retinal pigment epithelium (RPE) and pancreas, while expression of FRP-1,2 and 4 is high in developing tissues.
The FRPs/SARPs are involved in the Wnt signaling pathway by regulating the intracellular levels of beta-catenin.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human FRZB. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human FRZB.
