FOXO1 + FOX3 + FOX4 belong to the forkhead family of transcription factors.
FOXO1 (FKHR or ForkHead in Rhabdomyosarcoma) is a 70kD protein.
Recent studies have shown that this protein can act as either a coactivator or a corepressor of nuclear receptor activity.
This action is mediated through the LXXLL motif found in the C-terminal of the FOXO1A protein.
The specific function of the gene has not yet been determined.FOX3 probably functions as a trigger for apoptosis through expression of genes necessary for cell death.
Translocation of the FOXO3 gene with the MLL gene is associated with secondary acute leukemia.
FOX4 is a forkhead transcription factor involved in the regulation of the insulin signaling pathway.
It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1.
FOXO4 down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes.
It is also involved in negative regulation of the cell cycle.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes human FOXO1/O3. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa290-335 of human FOXO1/O3.
