84-4385-67　［取扱停止］FLOT2 (Flotillin-2, Epidermal Surface Antigen, ESA, Membrane Component Chromosome 17 Surface Marker 1, ESA1, M17S1) 100ul　360256

特徴

• Lipid rafts are sphingolipid- and cholesterol-rich membrane microdomains that are insoluble in nonionic detergents.
• Lipid rafts are important for numerous cellular processes, including signal transduction, membrane trafficking and molecular sorting.
• Flotillins are lipid raft components in neurons and caveloae-associated proteins in A498 kidney cells.
• Flotillin-2, also designated epidermal surface antigen, is conserved in all mammalian species.
• Flotillin-1 and -2 have complementary tissue distributions and their expression levels are independently regulated.
• At the cellular level, Flotillin-2 is ubiquitously expressed, whereas Flotillin-1 is expressed in A498 kidney cells, muscle cell lines and fibroblasts.
• Stable transfection of a Flotillin-2 fusion protein in COS cells induces filopodia formation and changes epithelial cells to a neuronal appearance.
• Flotillins form a ternary complex with CAP and Cbl, directing the localization of the CAP-Cbl complex to a lipid raft subdomain of the plasma membrane.
• Association of ER-X with Flotillin localizes ER-X within plasma membrane caveloae and mediates rapid oestrogen activation of the MAP kinase cascade.
• The expression of the flotillins is also correlated to the progression of Alzhemier pathology.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.