84-4385-13　［取扱停止］FADD (FAS-Associated Death Domain Protein, FAS-associating Death Domain-containing Protein, Growth-inhibiting Gene 3 Protein, Mediator of Receptor induced Toxicity, Protein FADD, MORT1, GIG3) 100ul　360217

特徴

• In contrast to growth factors which promote cell proliferation, FAS ligand (FAS-L) and the tumor necrosis factors (TNFs) rapidly induce apoptosis.
• Cellular response to FAS-L and TNF is mediated by structurally related receptors containing a conserved “death domain” and belonging to the TNF receptor superfamily.
• TRADD, FADD and RIP are FAS/TNF-R1 interacting proteins that contain a death domain homologous region (DDH).
• TRADD (TNF-R1-associated death domain) and FADD (FAS-associated death domain) associate with the death domains of both FAS and TNF-R1 via their DDH regions.
• Overexpression of TRADD leads to NFKB activation and apoptosis in the absence of TNF.
• Overexpression of FADD causes apoptosis, which can be blocked by the cow pox protein CrmA, suggesting that FADD lies upstream of ICE and possibly other serine proteases.
• The receptor interacting protein, RIP, associates with FAS exclusively via its DDH and this association is abrogated in lpr mutants.
• Unlike TRADD and FADD, RIP contains a putative amino-terminal kinase domain.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:500-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.