A gene on chromosome 8q13.3 encodes EYA1 (eyes absent), a protein with 16 exons.
EYA1 is one of four members of the eyes absent family.
A 27aa domain at the C-terminal is highly conserved amongst the members of the eyes absent family, while the PST (proline-serine- threonin)-rich N-terminal is highly divergent.
EYA is expressed in flexor tendons and the developing central nervous system, kidney, eye and ear.
EYA1 acts a transcriptional activator in connective tissue patterning through its PST domain, which functions as a transactivation domain.
EYA1 plays a critical role in the development of the inner ear and kidney.
EYA is involved in early inductive signaling, acting upstream of GDNF.
EYA1 has been implicated in the autosomal dominant disorders branchio-oto-renal (BOR) syndrome and branhio-oto (BO) syndrome.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human EYA1/4. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to residues in human EYA1/4.
