The Ets-1 family of transcription factors has a conserved DNA binding domain through which it plays an important role in cellular proliferation, differentiation, tematopoiesis and angiogenesis.
This domain, also known as the Ets domain, binds to DNA sequences containing the consensus sequence 5'-WGGA-3', which is known as the Ets-binding domain.
Elf-4, also known as myeloid Elf-1-like factor, ELF4 or MEF, is a 663aa member of the Ets-1 family.
Localized to the nucleus, Elf-4 is highly expressed in placenta and myeloid leukemia cells, with lower levels of expression lung, heart, thymus, slpeen, colon, ovary and peripheral blood lymphocytes.
Functioning primarily to activate the promoters of hematopoietic growth factor genes, such as GM-CSF, IL-3 and IL-8, Elf-4 has also been shown to activate the Perforin 1 promoter in natural killer (NK) cells, suggesting a possible role in tumorigenesis.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:50-1:200Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human ELF4.
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human ELF4.
