In mammalian cells, translation is controlled at the level of polypeptide chain initiation by initiation factors.
The eukaryotic translation initiation factor 5 (eIF5) catalyzes the hydrolysis of GTP bound to the 40S ribosomal subunit, a function necessary for the subsequent joining of the 40S and 60S subunits to form the 80S initiation complex.
eIF5B (Eukaryotic translation initiation factor 5B), also known as Translation initiation factor IF-2, is a 1120aa cytoplasmic protein that functions in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes.
eIF5B interacts with Annexin V, an anticoagulant protein, in a calcium and phospholipid-dependent manner.
Since eIF5B is conserved among all three kingdoms of life, it is also known as an universal initiation factor.
Applications:Suitable for use in Immunofluorescence, Western Blot, and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:50-1:200Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human EIF5B. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human EIF5B.
