In mammalian cells, translation is controlled at the level of polypeptide chain initiation by initiation factors.
The eukaryotic translation initiation factor 5 (eIF5) catalyzes the hydrolysis of GTP bound to the 40S ribosomal subunit, a function necessary for the subsequent joining of the 40S and 60S subunits to form the 80S initiation complex.
eIF-4E specifically binds to the mRNA cap to promote unwinding and exposure of the AUG-initiation codon.
Overexpression of eIF-4E can lead to cell transformation and tumorigenesis.
An additional initiation factor, eIF-2, is present as a heterotrimer composed of eIF-2a, eIF-2b and eIF-2g subunits.
This heterotrimer forms a complex with GTP and tRNA which then binds to the 40S ribosomal subunit.
After the formation of the 80S initiation complex, eIF-2 is hydrolyzed and eIF-2-GDP is released from the complex.
eIF-2-GDP is subsequently converted to eIF-2-GTP, a reaction catalyzed by eIF-2B, and is then available to catalyze another round of initiation.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human EIF5. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human EIF5.
