EF-Ts, also known as TSFM (Ts translation elongation factor, mitochondrial) or COXPD3, is a 325aa protein that is one of 13 mitochondrial-encoded proteins that work together during the elongation phase of protein biosynthesis on the ribosome.
Expressed ubiquitously with highest levels present in liver, kidney and skeletal muscle, EF-Ts associates with EF-Tu, a multidomain GTPase with essential functions in translation, and, via this interaction, facilitates the exchange of GDP for GTP, thereby inducing protein elongation.
Mutations in the gene encoding EF-Ts are the cause of combined oxidative phosphorylation deficiency type 3 (COXPD3), a condition characterized by defects in the mitochondrial oxidative phosphorylation system and often characterized by severe metabolic acidosis with encephalomyopathy or with hypertrophic cardiomyopathy.
Multiple isoforms of EF-Ts exist due to alternative splicing events.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:50-1:200Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human EF-Tu.
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human EF-Tu.
