The human endothelins represent a gene family comprised of endothelin-1, endothelin-2, and endothelin-3, also known as ET-1, ET-2, and ET-3.
Endothelins can affect the central nervous system and neuronal excitability, and they elicit potent vasoconstrictor action.
The two receptor subtypes responsible for inducing vasoconstriction and vasodilation, ETA and ETB, have different receptor affinities for ET-1, ET-2, and ET-3.
Of the three isopeptides, ET-2 has the most potent vasoconstrictor activity.
Biologically active ETs are proteolytically generated from a larger precursor, the big-endothelin, by action of the endothelin-converting enzyme (ECE) family.
ET-1 is a potent, 21aa vasoconstrictor peptide produced by vascular endothelial cells.
The ET-2 cDNA is 1.3kb in length and encodes a proprotein consisting of 178aa residues.
ET3 mRNA encodes a 230aa precursor that includes ET3 and a 15aa homologous segment called the ET3-like sequence.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes human EDN1. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to human EDN1.
