84-4383-95　［取扱停止］DYNLL2 (Dynein Light Chain 2, Cytoplasmic, 8kD Dynein Light Chain b, DLC8b, Dynein Light Chain LC8-type 2, DLC2) 100ul　360136

特徴

• Dyneins are multisubunit, high molecular weight ATPases that interact with microtubules to generate force by converting the chemical energy of ATP into the mechanical energy of movement.
• Cytoplasmic or axonemal Dynein heavy, intermediate, light and light-intermediate chains are all components of minus end-directed motors; the complex transports cellular cargos towards the central region of the cell.
• The highly conserved DYNLL proteins were originally identified as light chains for microtubule-based motor protein Dynein.
• In mammals there are two closely related isoforms expressed, DYNLL1 and DYNLL2 which share 93% sequence identity at the protein level.
• DYNLL1 (Dynein light chain 1) also designated, DLC8 or PIN (Protein inhibitor of neuronal nitric oxide synthase) has been identified as a protein that interacts with NOS1 resulting in NOS1 inhibition.
• Dimerization is required for NOS1 activity and DYNLL1 has been shown to destabilize the NOS1 dimer.
• Nitric oxide may be involved in several processes such as apoptosis, synaptogenesis and neuronal development; thus DYNLL1 is implicated in these processes as well.
• DYNLL1 is a ubiquitously expressed protein that exhibits high expression in testis and moderate expression in brain.
• DYNLL2 (Dynein light chain 2) is subject to a unique alternative splicing event which is implicated in Myosin Va binding specificity.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.