Diacylglycerol kinases (DGKs) phosphorylate diacylglycerol (DAG) to produce phosphatidic acid.
DAG and phosphatidic acid are lipids that act as second messengers in signaling cascades.
DGK-alpha influences cell activation and secretion of lethal exosomes, which in turn control cell death.
DGK-beta is abundant in restricted brain regions such as the caudate putamen and olfactory tubercle.
DGK-gamma encodes full-length and truncated transcripts that are present in a range of human tissues, with greatest expression observed in retina.
DGK-delta is most abundant in skeletal muscle.
DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol and is expressed predominantly in testis.
DGK-zeta is most abundant in brain and muscle.
DGK-eta is closely related to DGK-delta.
DGK-theta is most abundant in the cerebellum and hippocampus.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:50-1:200Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human DGK-a. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human DGK-a.
