The Ced/ICE or caspase family of cysteine proteases plays a pivotol role in mediating apoptosis through the proteolysis of specific targets.
Among the targets are poly(ADP-ribose) polymerase (PARP), Gelsolin, ICAD (inhibitor of CAD, also designated DFF-45) and the nuclear lamins.
CAD (caspase-activated deoxyribonuclease), also designated CPAN (caspase-activated nuclease) and DFF40, is a DNase that is responsible for DNA degradation during apoptosis.
Caspase-3 acts to dissociate CAD from ICAD, allowing CAD to enter the nucleus and degrade DNA.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human DFFB. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human DFFB.
