Long chain acyl-CoA esters (LCAs) act as both substrates and intermediates in metabolism, and as regulators of various intracellular functions.
Acyl-CoA binding protein (ACBP) specifically binds to LCA with high affinity and regulates its availability.
ACBP is structurally and functionally conserved among a diverse group of organisms, including human, rat, frog, insect, plant and yeast.
DBI, the gene encoding human ACBP, which maps to chromosome 2, is highly expressed in liver, soleus muscle and heart.
The ACBP protein is also abundant in cells with a high level of lipogenesis and de novo fatty acid synthesis.
Expression of ACBP is significantly induced during adipocyte differentiation.
DBI is a target gene for proliferator-activated receptor (PPAR) gamma, and is directly activated by PPARgamma/RXRalpha; and PPARalpha/RXRalpha; but not by PPARdelta/ RXRalpha;.
In addition to acyl-CoA binding and transport, ACBP is also implicated in g-aminobutyric acid type A receptor binding, steroidogenesis and peptide hormone release.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes human DBI. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human DBI.
