Aminoacyl-tRNA synthetases consist of a family of enzymes that catalyze the specific aminoacylation of cognate tRNA in the initial step of ribosome-dependent protein biosynthesis.
DaRS is part of a multisubunit complex of aminoacyl-tRNA synthetases and is involved in the transfer of Asp-tRNA to EF-1 alpha1 (elongation factor alpha 1).
The N-terminal of DaRS in vertebrates is a newly evolved structure that contains a putative amphiphilic helix and is dissimilar between different species.
The N-terminal extension acts as a switch that, when in its stretched form, reduces the rate of dissociation of Asp-tRNA from DaRS, thereby providing enough time for EF-1 alpha1 to interact with Asp-tRNA.
This suggests that the N-terminal of DaRS plays a critical role in its catalytic function.
DaRS contains two phosphorylations sites, forms homodimers and localizes to the cytoplasm.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human DARS. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human DARS.
