Fragile X syndrome is the most frequent form of inherited mental retardation and is a result of transcriptional silencing of the FMR1 gene on the X chromosome.
The FMR1 protein (also designated FMRP) is an RNA-binding protein that associates with polyribosomes and is a likely component of a messenger ribonuclear protein (mRNP) particle.
FMR1 can also interact with two fragile X syndrome related factors, FXR1 (also designated FXR1P) and FXR2 (also designated FXR2P).
These proteins form heterodimers through their N-terminal coiled-coiled domains.
CYFIP1 and CYFIP2 (also known as cytoplasmic FMRP interacting proteins 1 and 2, respectively, and as Sra-1 in mouse) both interact with FMR1 but CYFIP2 also reacts with FXR1 and FXR2.
CYFIP1 and CYFIP2 bind GTP-bound Rac1 to release FMRP in its active state, which is thought to regulate mRNA translation of neural cytoskeletal proteins.
A loss of CYFIP1 and CYFIP2 leads to mutant neurons with defective axonal growth and motor function.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human CYFIP1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues in human CYFIP1.
