Cathepsin W (lymphopain) and cathepsin F comprise a novel subgroup of cathepsin proteases, and are phylogenetically distinct from other human cathepsins.
The cathepsin W gene maps to chromosome 11q13.1 and contains ten exons with introns ranging from 81-119 bp.
Cathepsin W protein is expressed specifically in CD8+ T lymphocytes.
The expression of cathepsin W first occurs during the differentiation of thyrocytes to CD8+ T lymphocytes, just as the thymocytes cease expression of CD4+ receptors.
In transfected Cos-7 and HeLa cells, cathepsin W localizes within the rough endoplasmic reticulum.
Cathepsin W contains a unique 21aa peptide insertion between the active site histidine and asparagine residues, in addition to a distictive 8aa carboxy-terminal extension.
An extended loop structure in the second or beta-sheet domain and an additional disulfide bind are two of several signature features of cathepsin W.
Other features of cathepsin W include an additional cysteine, an S2 pocket and an additional residue.
Cathepsin W may exist as a dimer with each monomer forming a disulfide bond.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human CTSW. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues in human CTSW.
