CTH (cystathionine gamma-lyase), also known as CSE or gamma-cystathionase, is a member of the trans-sulfuration enzyme family and participates in the trans-sulfuration pathway.
CTH is a cytoplasmic enzyme produced in the cytosol and is responsible for catalyzing the pyridoxal phosphate-dependent b-disulfide elimination reaction resulting in ammonium, pyruvate and thiocysteine.
The thiocysteine that is produced may then react with other thiols (or cysteine) and form hydrogen sulfide (H2S).
Thus, CTH is the major H2S-producing enzyme in kidney, liver, vascular smooth muscle cells and enterocytes.
The endogenous production of H2S plays a significant role in the regulation of cellular functions, including cell growth, hyperpolarization of cell membranes, modulation of neuronal excitability and relaxation of smooth muscle cells.
Mutations in the gene encoding CTH can result in the autosomal recessive disease cystathioninuria; a disorder characterized by the unusual accumulation of plasma cystathionine causing increased urinary excretion.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human CTH. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues in human CTH.
