Granulocyte-colony stimulating factor, G-CSF, is a pleiotropic cytokine that influences differentiation, proliferation and activation of the neutrophilic granulocyte lineage.
The murine G-CSF cDNA encodes a 208aa precursor containing a 30aa signal peptide that is proteolytically cleaved to form a 178aa residue mature protein.
Two G-CSF cDNAs, which are identical except for a 3aa deletion in the N-terminus of one form of the protein, have been isolated from human cells.
Murine and human G-CSF share 73% sequence identity at the amino acid level.
G-CSF signals through the G-CSF receptor, G-CSFR, a heavily glycosylated 812aa polypeptide with a single transmembrane domain.
Stimulation of the G-CSFR results in the activation of the Ras/MAPK pathway and phosphorylation of the adaptor protein Shc.
Studies indicate that the kinases Lyn and Syk and the transcription factor Stat3 are activated in response to G-CSF stimulation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human CSF3. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human CSF3.
