Src homology (SH2) domains are non catalytic sequences that are conserved among a number of cytoplasmic signaling proteins.
These signaling proteins are directly regulated by receptor tyrosine kinases and control the activation of mitogenic signal transduction pathways by such receptors.
For instance, ligand-induced activation of the EGF and PDGF receptors induces dimerization, triggers receptor autophosphorylation on tyrosine residues and results in the binding of a number of cytoplasmic SH2 domain proteins such as PLC-g1, Ras GAP and PI 3-kinase p85 to the activated receptors.
The Shc gene encodes proteins with a single SH2 domain but no identifiable catalytic domain.
CIS, cytokine-inducible SH2-containing protein, is a 267aa protein with a single 96aa SH2 domain that associates the tyrosine- phosphorylated beta chain of the IL-3 receptor with the tyrosine-phosphorylated Epo receptor.
CIS was initially described as an immediate early cytokine-responsive gene and appears to be a unique regulator of cytokine signaling.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.5, 0.05% sodium azide.
Specificity：Recognizes human CISH. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human CISH.
