Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs and xenobiotic compounds.
These enzymes differ in their tissue distributions and substrate specificities, although the gene structure (number and length of exons) is similar among family members.
Carbohydrate sulfotransferase 5 (CHST5) (also referred to as GlcNAc6ST-3 or IGn6ST) and carbohydrate sulfotransferase 6 (CHST6) (also referred to as GlcNAc6ST-5 or Cgn6ST) are predominantly expressed in the intestine and cornea, respectively.
They are highly homologous and both are orthologs of the murine CHST5.
CHST5 and CHST6 may be the result of gene duplication.
They catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues.
CHST5 preferably mediates the sulfation of short carbohydrates and O-linked sugars of mucin-type acceptors.
CHST6 mediates the sulfation of keratan in the cornea, which is important in maintaining corneal transparency.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes human CHST6. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human CHST6.
