The normal progression of cells through the cell cycle is under the control of the cyclin dependent protein kinases Cdk4 and Cdk6, which are subject to inhibition by the mitotic inhibitory protein, p16.
Isolated members of the p16 family have been designated p15, p18 and p19.
p15 expression is upregulated approximately 30-fold in TGF beta-treated human keratinocytes, suggesting that p15 may function as an effector of TGF beta-mediated cell cycle arrest through inhibition of Cdk4 and Cdk6 kinases.
The gene encoding p15 has been mapped to chromosome 9p21.3 at a position adjacent to the p16 gene, at a site of frequent chromosomal abnormality in human tumors.
Two p16- related proteins, p19 and p18, specifically inhibit the kinase activities of Cdk4 and Cdk6 but do not affect those of cyclin E-Cdk2, cyclin A-Cdk2 or cyclin B-Cdc2 complexes.
p19 is expressed at maximal level during S phase, while overexpression of p19 leads to G1 arrest.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human CDKN2D.
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human CDKN2D.
