84-4380-73　［取扱停止］CD274 (Programmed Cell death 1 Ligand 1, PD-L1, PDCD1 Ligand 1, Programmed Death Ligand 1, B7 Homolog 1, B7-H1, CD274, B7H1, PDCD1L1, PDCD1LG1, PDL1) 50ul　359960

特徴

• Engagement of CD28 by B7-1 (CD80) or B7-2 (CD86) in the presence of antigen promotes T cell proliferation, cytokine production, differentiation of effector T cells, and the induction of Bcl-x, a promoter of T cell survival.
• Conversely, engagement of CTLA4 by B7-1 or B7-2 may inhibit proliferation and IL-2 production.
• Pdcd-1L1 (programmed cell death ligand-1), also known as B7-H1 or PD-L1, is 290 amino acid type I transmembrane protein which is 20% and 15% identical to B7-1 and B7-2, respectively.
• Pdcd-1L2 has immunoglobulin V-like and C-like domains and a 30 amino acid cytoplasmic tail.
• It does not bind CD28, cytotoxic T-lymphocyte A4 or ICOS (inducible co-stimulator).
• IL-2, although produced in small amounts, is required for the effect of Pdcd-1L1 co-stimulation.
• The gene which encodes Pdcd-1L1 maps to human chromosome 9p24.
• Pdcd-1L2 (programmed cell death ligand-2) is a 73aa protein which contains a signal sequence, IgV- and IgC-like domains, a transmembrane region and a cytoplasmic region.
• The gene which encodes Pdcd-1L2 maps to human chromosome 9p24.2.
• The constitutive expression of Pdcd-1L1 and Pdcd-1L2 on paren-chymal cells of heart, lung and kidney suggests that the Pdcd-1-Pdcd-L system could provide unique negative signaling to help prevent autoimmune disease.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.