84-4380-71　［取扱停止］CD247 (T-Cell Surface Glycoprotein CD3 zeta Chain, T-Cell Receptor T3 zeta Chain, CD247, CD247, CD3Z, T3Z, TCRZ) 50ul　359959

特徴

• The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state.
• Much of this signaling process can be attributed to a multisubunit complex of proteins that associates directly with the TCR.
• This complex has been designated CD3 (cluster of differentiation 3).
• It is composed of five invariant polypeptide chains that associate to form three dimers: a heterodimer of gamma and epsilon chains (ge), a heterodimer of delta and epsilon chains (de) and a homodimer of two zeta chains (ZZ) or a heterodimer of zeta and eta chains (Zh).
• The Z and h chains are encoded by the same gene but differ in their C-terminal ends due to an alternative splicing event.
• The g, e and d chains each contain a single copy of a conserved immunoreceptor tyrosine-based activation motif (ITAM).
• In contrast, the Z chain contains three consecutive copies of the same motif.
• Phosphorylated ITAMs act as docking sites for protein kinases such as ZAP-70 and Syk and are also capable of regulating their kinase activity.
• The crystal structure of the ZAP-70 SH2 domains bound to the Z chain ITAMs has been solved.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.