Langerhans cells (LCs) are a subset of immature dendritic cells (DCs) that specifically localize in the epidermis and other mucosal epithelia.
Epidermal LCs possess strong immunostimulatory capacity and play a central role in the initiation and regulation of immune responses.
Langerin (CD207) is a Ca(++)-dependent, C-type lectin domain-containing, type II transmembrane protein that induces epidermal LCs to differentiate into Birbeck granules (BG).
BGs are organelles with superimposing and zippering membranes that influence proper class I type antigen presentation to the circulating T-cells.
Human spleen, lymph node, thymus, liver, lung and heart express langerin protein.
The human langerin gene maps to chromosome 2p13 and encodes a 328aa protein.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human CD207. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to human CD207.
