Centrosome duplication and separation are linked inextricably to certain cell cycle events, specifically, activation of cyclin-dependent kinases of cyclin-dependent kinases.
CP110 (centrosomal protein of 110kD) is a 991aa cell cycle-dependent CDK substrate that regulates centrosome duplication.
Localizing to the centrosome, CP110 contains ten putative CDK2 phosphorylation sites, two cyclin-binding domains and two degradation motifs.
CP110 is highly expressed in testis with much lower expression in all other tissues.
CP110 interacts with Ca2+-binding proteins including calmodulin (CaM) and centrin, to regulate genome stability and progression through cytokinesis.
During the formation of cylindrical centrioles, it is suggested that CP110 acts as a distal end-capping protein thereby limiting the elongation of newly formed centrioles.
Existing as two alternatively spliced isoforms, CP110 is observed at highest levels during the S phase of the cell cycle.
CP110 becomes phosphorylated by Cdks (cyclin-dependent kinases) and is encoded by a gene located on human chromosome 16p12.3.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes human CCP110.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human CCP110.
