Chemokines have been implicated in the regulation of stem/progenitor cell proliferation and movement.
C-C chemokines myeloid progenitor inhibitory factor-1 (MPIF)-1 and eotaxin-2 (also known as MPIF-2, CK b-6 or small inducible cytokine A24) both map to chromosome 7q11.23.
MPIF-1 has chemotactic activity on dendritic cells derived from either peripheral blood monocytes or cord blood CD34+ progenitors.
It is also a potent suppressor of bone marrow low proliferative potential colony-forming cells.
Eotaxin-2, which promotes chemotaxis and Ca2+ mobilization in human eosinophils, exerts its activity solely through the CCR3 receptor.
In addition, eotaxin-2 lacks suppressive activity against immature subsets of myeloid progenitors, which have been stimulated to proliferate by multiple growth factors.
A related C-C chemokine, eotaxin-3, shares only 33% amino acid identity with eotaxin-2, but shares many characteristics with eotaxin-2.
Eotaxin-3 induces migration of eosinophils and basophils at a 10-fold higher concentration than eotaxin-2.
The gene which encodes eotaxin-3 maps to human chromosome 7q11.23.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.
Specificity：Recognizes human CCL26. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human CCL26.
