Membrane-associated guanylate kinase (MAGUK) family members localize to the plasma membrane and function as molecular scaffolds for the assembly of multi-protein complexes.
The MAGUK family includes several mammalian proteins related to the Drosophila tumor suppressor discs-large (dlg) gene product, such as postsynaptic proteins, GKAPs, the tight junction associated proteins (ZO-1–3) and the caspase-associated recruitment domain (CARD) proteins: CARD 6, CARD 8-12 and CARD 14.
CARD 14 is a 1004aa protein consisting of an N-terminal CARD domain, a central coiled-coil domain and a C-terminal tripartite domain comprised of a PDZ domain, an Src homology 3 domain and a GUK domain with homology to guanylate kinase.
CARD 14 is expressed in the placenta where it positively regulates apoptosis.
CARD 14 also controls NF˚B activation by phosphorylating BCL10, a signaling protein that activates NF˚B through the I˚B kinase complex.
Epigallocatechin-3-gallate (EGCG) is a polyphenol that induces the expression of CARD 14.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human CARD14. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human CARD14.
