The Calpain family of proteins are calcium-regulated thiol proteases which have broad endopeptidase activity throughout the body.
Calpain small subunit 2, also known as CSS2 or CAPNS2, is a calcium-dependent protease that is expressed ubiquitously in the cytoplasm.
Part of a heterodimer composed of a small subunit and a large subunit, CSS2 catalyzes proteolysis of various proteins involved in cytoskeletal remodeling and signal transduction.
CSS2 also acts as a chaperone to the larger subunit, mediating its correct folding and conformation.
When bound as a heterodimer, CSS2 is thought to keep the catalytic activity of the large subunit dormant.
After binding calcium, CSS2 is released from the complex, thereby activating the large subunit and allowing CSS2 to translocate from the cytoplasm to the cell membrane.
Defects in the gene encoding CSS2 result in incorrect calpain activity and retarded fetal development, suggesting that CSS2 expression is essential for proper growth.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human CAPNS2. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues in human CAPNS2.
