Calpain, an intracellular calcium-dependent protease that cleaves cytoskeletal and submembranous proteins, plays a role in cytoskeletal reorganization and muscle protein degradation.
Calpain is a heterodimer composed of a small regulatory subunit and one of three large catalytic subunits, designated calpain 1, calpain 2 and calpain 3.
Calpain 3 (calpain p94) is a muscle-preferred calcium activated neutral protease that localizes to the nucleus.
The gene encoding human calpain 3 maps to chromosome 15q15.1-q21.1.
Mutations involving the calpain 3 gene are associated with limb-girdle muscle dystrophy type 2A, a form of autosomal recessive and progressive neuromuscular disorder.
Calpastatin regulates calpain by inhibiting both the proteolytic activity of calpain and its binding to membranes.
Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes human CAPN3. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to residues in human CAPN3.
