Excitable cells in response to membrane depolarization are involved in a variety of Ca++-dependent processes, including muscle contraction, hormone or neurotransmitter release and gene expression.
Calcium channels are highly diverse, multimeric complexes composed of an alpha1 subunit, an intracellular beta subunit, a disulfide linked alpha2/delta subunit and a transmembrane gamma subunit.
L-type Ca++ currents initiate muscle contraction, endocrine secretion and gene transcription, and are regulated through second-messenger activated protein phosphorylation pathways.
L-type calcium channels may form macromolecular signaling complexes with G protein-coupled receptors, thereby enhancing the selectivity of regulating specific targets.
L-type calcium channels in the brain specifically express the gamma2 subunit along with gamma3 and gamma4 subunits.
The gamma2 subunit (also known as stargazin) is abundant in synaptic plasma membranes where it regulates synaptic targeting of AMP receptors in granule cells.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human CACNG2.
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human CACNG2.
