The transcription factor C/EBP a (CCAAT-enhancer binding protein) is a heatstable, sequence-specific DNA-binding protein first purified from rat liver nuclei that binds avidly to several different cis-regulatory DNA sequences commonly associated with viral and cellular genes transcribed by RNA polymerase II.
C/EBPalpha regulates gene expression in a variety of tissues including liver, adipose, lung and intestine.
C/EBPalpha uses a bipartite structural motif to bind DNA.
Two protein chains dimerize through a set of amphipathic alpha helices termed the leucine zipper.
Highly basic polypeptide regions emerge from the zipper to form a linked set of DNA contact surfaces.
C/EBP alpha appears to function exclusively in terminally differentiated, growth-arrested cells.
Additional family members include C/EBPB, C/EBP g,C/EBPd and C/EBP e, all of which exhibit similar DNA-binding specificities and affinities to C/EBP a Furthermore, C/EBP B and C/EBP d readily form heterodimers both with each other as well as with C/EBP a.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:50-1:200Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human C/EBP-gamma. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human C/EBP-gamma.
