BLM hydrolase (Bleomycin hydrolase, BLM hydrolase, BMH) is a 455aa protein encoded by the human gene BLMH.
BLM hydrolase belongs to the cysteine protease papain superfamily and the peptidase C1 family.
It is a cytoplasmic cysteine peptidase commonly found as a homohexamer.
It is highly conserved through evolution, however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM).
BLM is an essential component of combination chemotherapy regimens for cancer.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human BLMH.
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human BLMH.
